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Immunogenomic engineering of a plug-and-(dis)play hybridoma platform

17 Aug 2016

Abstract excerpt

Hybridomas, fusions of primary mouse B cells and myelomas, are stable, rapidly-proliferating cell lines widely utilized for antibody screening and production. Antibody specificity of a hybridoma clone is determined by the immunoglobulin sequence of the primary B cell. Here we report a platform for rapid reprogramming of hybridoma antibody specificity by immunogenomic engineering. Here we use CRISPR-Cas9 to...

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PMID: 27531490
DOI: 10.1038/ncomms12535

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