Article

Functional diversification of hybridoma produced antibodies by CRISPR/HDR genomic engineering

2019-02-15

Abstract excerpt

Hybridoma technology is instrumental for the development of novel antibody therapeutics and diagnostics. Recent preclinical and clinical studies highlight the importance of antibody isotype for therapeutic efficacy. However, since the sequence encoding the constant domains is fixed, tuning antibody function in hybridomas has been restricted. Here, we demonstrate a versatile CRISPR/HDR platform to rapidly engineer...

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Literature Corpus work: a17dab2c-0f9d-5d32-916f-393c7153539f
DOI: 10.1101/551382

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