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Reprogramming the antigen specificity of B cells using genome-editing technologies

16 Jan 2019

Abstract excerpt

We have developed a method to introduce novel paratopes into the human antibody repertoire by modifying the immunoglobulin (Ig) genes of mature B cells directly using genome editing technologies. We used CRISPR-Cas9 in a homology directed repair strategy, to replace the heavy chain (HC) variable region in B cell lines with that from an HIV broadly neutralizing antibody (bnAb), PG9. Our strategy is designed to...

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PMID: 30648968
DOI: 10.7554/elife.42995

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