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Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease

8 Aug 2018

Abstract excerpt

Abstract B cells offer unique opportunities for gene therapy because of their ability to secrete large amounts of protein in the form of antibody and persist for the life of the organism as plasma cells. Here, we report optimized CRISPR/Cas9 based genome engineering of primary human B cells. Our procedure involves enrichment of CD19 + B cells from PBMCs followed by activation, expansion, and electroporation of...

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PMID: 30108345
DOI: 10.1038/s41598-018-30358-0

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