Article

Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease

2018-03-28

Abstract excerpt

B cells offer unique opportunities for gene therapy because of their ability to secrete large amounts of protein in the form of antibody and persist for the life of the organism as plasma cells. Here, we report optimized CRISPR/Cas9 based genome engineering of primary human B cells. Our procedure involves enrichment of CD19 + B cells from PBMCs followed by activation, expansion, and electroporation of CRISPR/Cas9...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a2b0bd72-b452-5a48-8cf0-118a3692577d
DOI: 10.1101/290973

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Engineering of Primary Human B cells with CRISPR/Cas9 Targeted NucleaseDOI 10.1101/290973Read

Engineering of Primary Human B cells with CRISPR/Cas9 Targeted NucleaseSemantic proximity Read

Genetic engineering in primary human B cells with CRISPR-Cas9 ribonucleoproteins.Semantic proximity Read

Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells.Semantic proximity Read

CRISPR-Mediated Editing of the B Cell Receptor in Primary Human B CellsSemantic proximity Read

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infectionSemantic proximity Read

An arrayed CRISPR screen of primary B cells reveals the essential elements of the antibody secretion pathwaySemantic proximity Read

Reprogramming the antigen specificity of B cells using genome-editing technologiesSemantic proximity Read

Reprogramming the antigen specificity of B cells using genomeediting technologiesSemantic proximity Read

Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturingSemantic proximity Read

Molecular Design, Optimization, and Genomic Integration of Chimeric B Cell Receptors in Murine B CellsSemantic proximity Read

Generating universal anti-CD19 CAR T cells with a defined memory phenotype by CRISPR/Cas9 editing and safety evaluation of the transcriptome.Semantic proximity Read

Efficient Gene Editing of CART Cells with CRISPR-Cas12a for Enhanced Antitumor EfficacySemantic proximity Read