Article

Superloser: a plasmid shuffling vector for <i>Saccharomyces cerevisiae</i> with exceedingly low background

2019-05-07

Abstract excerpt

Here we report a new plasmid shuffle vector for forcing budding yeast ( Saccharomyces cerevisiae ) to incorporate a new genetic pathway in place of a native pathway – even essential ones – while maintaining low false positive rates (less than 1 in 10 8 per cell). This plasmid, dubbed “Superloser”, was designed with reduced sequence similarity to commonly used yeast plasmids (i.e. pRS400 series) to limit recombin...

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Literature Corpus work: 36b8b4c6-e747-5cbe-91be-66214f52583c
DOI: 10.1101/630863

Open publication

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