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Study Essential Gene Functions by Plasmid Shuffling.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Fan Li, Xiao Wei

Abstract excerpt

An essential gene is defined as a gene that cannot be completely removed from the genome. Investigation of an essential gene function is limited because its deletion strain cannot be readily created. Here we describe a protocol called plasmid shuffling that can be conveniently employed in yeast to study essential gene functions. The essential gene is first cloned into a YCp-based plasmid with URA3 as a selectable...

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PMID: 32889712
DOI: 10.1007/978-1-0716-0868-5_5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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