Article

Rapid, scalable, combinatorial genome engineering by Marker-less Enrichment and Recombination of Genetically Engineered loci (MERGE)

2022-06-17

Abstract excerpt

Large-scale genome engineering in yeast is feasible primarily due to prodigious homology-directed DNA repair (HDR), a plethora of genetic tools, and simple conversion between haploid and diploid forms. However, a major challenge to rationally building multi-gene processes in yeast arises due to the combinatorics of combining all of the individual edits into the same strain. Here, we present an approach for scalabl...

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Literature Corpus work: 3fd23db2-0dc7-5e49-9156-5c4413adb955
DOI: 10.1101/2022.06.17.496490

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Rapid, scalable, combinatorial genome engineering by Marker-less Enrichment and Recombination of Genetically Engineered loci (MERGE)DOI 10.1101/2022.06.17.496490Read

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