Article
Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.
Yeast (Chichester, England) - 30 Jan 1998
Brachmann C B, Davies A, Cost G J, Caputo E, Li J, Hieter P, Boeke J D
Abstract excerpt
A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. Because the homology between commonly used...
Read the complete abstract on PubMedTopics
- Alleles
- Blotting, Northern
- Blotting, Southern
- DNA Primers
- Gene Deletion
- Gene Expression