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Article

Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage.

Genome research - 1 Mar 2011

Reid Robert J D, González-Barrera Sergio, Sunjevaric Ivana, Alvaro David, Ciccone Samantha, Wagner Marisa, Rothstein Rodney

Abstract excerpt

We have streamlined the process of transferring plasmids into any yeast strain library by developing a novel mating-based, high-throughput method called selective ploidy ablation (SPA). SPA uses a universal plasmid donor strain that contains conditional centromeres on every chromosome. The plasmid-bearing donor is mated to a recipient, followed by removal of all donor-strain chromosomes, producing a haploid...

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PMID: 21173034
DOI: 10.1101/gr.109033.110
PMCID: PMC3044861

Genes

TOP1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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