Article
Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
Methods in enzymology - 1 Jan 1991
Rothstein R
Abstract excerpt
The methods described in this chapter permit the manipulation of virtually any cloned yeast chromosomal sequence by virtue of the fact that DNA transformed into yeast integrates into the chromosome by homologous recombination. Furthermore, double-strand breaks in transforming DNA stimulate recomb...
Topics
- Alleles
- Cloning, Molecular
- DNA
- Genetic Markers
- Plasmids
- Restriction Mapping
- Saccharomyces cerevisiae
- Transformation, Genetic