Article
Manipulation of plasmids from yeast cells.
Current protocols in molecular biology - 1 May 2001
Lundblad V, Zhou H
Abstract excerpt
This unit describes several procedures for manipulating plasmids in yeast cells. The first is a general method to segregate autonomously replicating plasmids from cells: plasmid-containing yeast cells are grown in nonselective medium, and colonies lacking the plasmid are identified by replica plating. The second, plasmid shuffling, represents a specialized version of plasmid segregation that is useful for...
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