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Article

Plasmid-chromosome shuffling for non-deletion alleles in yeast.

Nature methods - 1 Feb 2008

Huang Zhiwei, Sucgang Richard S, Lin Yu-yi, Shi Xiaomin, Boeke Jef D, Pan Xuewen

Abstract excerpt

Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein...

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PMID: 18193055
DOI: 10.1038/nmeth.1173

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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