Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

High-efficiency genome editing and allele replacement in prototrophic and wild strains of Saccharomyces.

Genetics - 1 Nov 2014

Alexander William G, Doering Drew T, Hittinger Chris Todd

Abstract excerpt

Current genome editing techniques available for Saccharomyces yeast species rely on auxotrophic markers, limiting their use in wild and industrial strains and species. Taking advantage of the ancient loss of thymidine kinase in the fungal kingdom, we have developed the herpes simplex virus thymidine kinase gene as a selectable and counterselectable marker that forms the core of novel genome engineering tools...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chromosomes, Fungal
Share to Diploidy
Share to Genome, Fungal

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Homozygote

Share to Mutagenesis, Insertional

Share to RNA Editing

Share to Saccharomyces

Share to Thymidine Kinase

Source

Open in PubMed Open full text

Identifiers

PMID: 25209147
DOI: 10.1534/genetics.114.170118
PMCID: PMC4224175

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

High-efficiency genome editing and allele replacement in prototrophic and wild strains of Saccharomyces.PMID 25209147Read ↗

Designer deletion and prototrophic strains derived from Saccharomyces cerevisiae strain W303-1a.Semantic proximity Read ↗

Rapid, scalable, combinatorial genome engineering by Marker-less Enrichment and Recombination of Genetically Engineered loci (MERGE)Semantic proximity Read ↗

Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.Semantic proximity Read ↗

Construction of mutant alleles in Saccharomyces cerevisiae without cloning: overview and the delitto perfetto method.Semantic proximity Read ↗

A set of genetically diverged Saccharomyces cerevisiae strains with markerless deletions of multiple auxotrophic genes.Semantic proximity Read ↗

A recombinatorial method useful for cloning dominant alleles in Saccharomyces cerevisiae.Semantic proximity Read ↗

CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9.Semantic proximity Read ↗

Superloser: a plasmid shuffling vector for <i>Saccharomyces cerevisiae</i> with exceedingly low backgroundSemantic proximity Read ↗

In vivo continuous evolution of genes and pathways in yeast.Semantic proximity Read ↗

RNAi-assisted genome evolution in Saccharomyces cerevisiae for complex phenotype engineering.Semantic proximity Read ↗

Saccharomyces diversity and evolution: a budding model genus.Semantic proximity Read ↗

Conversion of polyploid and alloploid Saccharomyces sensu stricto strains to leu2 mutants by genome DNA editing.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.