Article
Total synthesis of a functional designer eukaryotic chromosome.
Science (New York, N.Y.) - 4 Apr 2014
Annaluru Narayana, Muller Héloïse, Mitchell Leslie A, Ramalingam Sivaprakash, Stracquadanio Giovanni, Richardson Sarah M, Dymond Jessica S, Kuang Zheng, Scheifele Lisa Z, Cooper Eric M, Cai Yizhi, Zeller Karen, Agmon Neta, Han Jeffrey S, Hadjithomas Michalis, Tullman Jennifer, Caravelli Katrina, Cirelli Kimberly, Guo Zheyuan, London Viktoriya, Yeluru Apurva, Murugan Sindurathy, Kandavelou Karthikeyan, Agier Nicolas, Fischer Gilles, Yang Kun, Martin J Andrew, Bilgel Murat, Bohutski Pavlo, Boulier Kristin M, Capaldo Brian J, Chang Joy, Charoen Kristie, Choi Woo Jin, Deng Peter, DiCarlo James E, Doong Judy, Dunn Jessilyn, Feinberg Jason I, Fernandez Christopher, Floria Charlotte E, Gladowski David, Hadidi Pasha, Ishizuka Isabel, Jabbari Javaneh, Lau Calvin Y L, Lee Pablo A, Li Sean, Lin Denise, Linder Matthias E, Ling Jonathan, Liu Jaime, Liu Jonathan, London Mariya, Ma Henry, Mao Jessica, McDade Jessica E, McMillan Alexandra, Moore Aaron M, Oh Won Chan, Ouyang Yu, Patel Ruchi, Paul Marina, Paulsen Laura C, Qiu Judy, Rhee Alex, Rubashkin Matthew G, Soh Ina Y, Sotuyo Nathaniel E, Srinivas Venkatesh, Suarez Allison, Wong Andy, Wong Remus, Xie Wei Rose, Xu Yijie, Yu Allen T, Koszul Romain, Bader Joel S, Boeke Jef D, Chandrasegaran Srinivasan
Abstract excerpt
Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair native Saccharomyces cerevisiae chromosome III. Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric...
Topics
- Base Sequence
- Chromosomes, Fungal
