Article

Minimized double guide RNA libraries enable scale-limited CRISPR/Cas9 screens

2019-11-29

Abstract excerpt

Genetic screens based on CRISPR/Cas technology are a powerful tool for understanding cellular phenotypes. However, the coverage and replicate requirements result in large experiment sizes, which are limiting when samples are scarce, or the protocols are expensive and laborious. Here, we present an approach to reduce the scale of genome-wide perturbation screens up to fivefold without sacrificing performance. To do...

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Literature Corpus work: 0b423a14-f20f-5cac-95c2-5c88fa0af748
DOI: 10.1101/859652

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