Article

Guide RNA library representation results in gene essentiality prediction bias in genome-wide CRISPR screens

2025-09-08

Abstract excerpt

Genome wide CRISPR-based perturbation screens are powerful discovery tools enabling the identification of novel gene dependencies through either gain or loss of function. While genome wide guide RNA (gRNA) libraries have advantages when using enAsCas12a, such as multiplex single gRNAs per gene, they may be subject to similar confounding factors that can affect the interpretation of large genome-wide datasets. Here...

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Literature Corpus work: 88a62d64-dfcc-5df3-abaa-3b548d04f760
DOI: 10.1101/2025.09.07.673082

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