Article
Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs.
Nature biotechnology - 1 Mar 2020
Jost Marco, Santos Daniel A, Saunders Reuben A, Horlbeck Max A, Hawkins John S, Scaria Sonia M, Norman Thomas M, Hussmann Jeffrey A, Liem Christina R, Gross Carol A, Weissman Jonathan S
Abstract excerpt
A lack of tools to precisely control gene expression has limited our ability to evaluate relationships between expression levels and phenotypes. Here, we describe an approach to titrate expression of human genes using CRISPR interference and series of single-guide RNAs (sgRNAs) with systematically modulated activities. We used large-scale measurements across multiple cell models to characterize activities of...
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