Article
Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones.
Stem cell research & therapy - 27 Apr 2023
Frank Elie, Cailleret Michel, Nelep Constantin, Fragner Pascal, Polentes Jérome, Herardot Elise, El Kassar Lina, Giraud-Triboult Karine, Monville Christelle, Ben M'Barek Karim
Abstract excerpt
BACKGROUND: CRISPR/Cas9 editing systems are currently used to generate mutations in a particular gene to mimic a genetic disorder in vitro. Such "disease in a dish" models based on human pluripotent stem cells (hPSCs) offer the opportunity to have access to virtually all cell types of the human body. However, the generation of mutated hPSCs remains fastidious. Current CRISPR/Cas9 editing approaches lead to a...
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