Article

A benchmark comparison of CRISPRn guide-RNA design algorithms and generation of small single and dual-targeting libraries to boost screening efficiency

2024-05-17

Abstract excerpt

Genome-wide CRISPR sgRNA libraries have emerged as transformative tools to systematically probe gene function. While these libraries have been iterated over time to be more efficient, their large size limits their use in some applications. Here, we benchmarked publicly available genome-wide single-targeting sgRNA libraries and evaluated dual targeting as a strategy for pooled CRISPR loss-of-function screens. We le...

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Literature Corpus work: 1dcc15c6-cc1a-5cfb-885d-be9f12ba1a8b
DOI: 10.1101/2024.05.17.594311

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