Article

Pooled CRISPR screening at high sensitivity with an empirically designed sgRNA library

2020-04-25

Abstract excerpt

<h4>ABSTRACT</h4> Given their broad utility in functionally annotating genomes, the experimental design of genome-scale CRISPR screens can vary greatly and criteria for optimal experimental implementation and library composition are still emerging. In this study, we report advantages of conducting viability screens in selected Cas9 single cell clones in contrast to Cas9 bulk populations. We further systematically...

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Literature Corpus work: c6e4e054-b96f-5092-9897-60d5a1fd8587
DOI: 10.1101/2020.04.25.061135

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