Article

Optimization of AsCas12a for combinatorial genetic screens in human cells

2019-08-28

Abstract excerpt

<h4>ABSTRACT</h4> Cas12a enzymes have attractive properties for scalable delivery of multiplexed perturbations, yet widespread usage has lagged behind Cas9-based strategies. Here we describe the optimization of Cas12a from Acidaminococcus (AsCas12a) for use in pooled genetic screens in human cells. By assaying the activity of thousands of guides, we confirm on-target design rules and extend them to an enhanced a...

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Literature Corpus work: 97b7157d-91d7-5623-aa6f-49a6208e34fa
DOI: 10.1101/747170

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