Article

Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation

2024-12-28

Abstract excerpt

CRISPR/Cas9 technologies provide unique capabilities for modeling disease and understanding gene-to-phenotype connections. In cultured cells, chemical-mediated control of Cas9 activity can limit off-target effects and enable mechanistic study of essential genes. However, widely-used Tet-On systems often show “leaky” Cas9 expression, leading to unintended edits, as well as weak activity upon induction. Leakiness ca...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d6752605-b0d2-5004-b6a8-592ab4707c0d
DOI: 10.1101/2024.12.27.630546

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbationDOI 10.1101/2024.12.27.630546Read

The CRISPR tool kit for genome editing and beyondSemantic proximity Read

Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future InnovationsSemantic proximity Read

Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translationSemantic proximity Read

Mutational rescue of the activity of high-fidelity Cas9 enzymes.Semantic proximity Read

CRISPRoff epigenetic editing for programmable gene silencing in human cells without DNA breaksSemantic proximity Read

Optimizing a CRISPR-Cas13d gene circuit for tunable target RNA downregulation with minimal collateral RNA cuttingSemantic proximity Read

CRISPR-Enabled Autonomous Transposable Element (CREATE) for RNA-based gene editing and deliverySemantic proximity Read

Massively Targeted Evaluation of Therapeutic CRISPR Off-Targets in CellsSemantic proximity Read

Precise transcript targeting by CRISPR-Csm complexesSemantic proximity Read

Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.Semantic proximity Read

Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging.Semantic proximity Read

License to cut: Smart RNA guides for conditional control of CRISPR-Cas9Semantic proximity Read