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Simple and reliable detection of CRISPR-induced on-target effects by qgPCR and SNP genotyping.

Nature protocols - 1 Mar 2021

Weisheit Isabel, Kroeger Joseph A, Malik Rainer, Wefers Benedikt, Lichtner Peter, Wurst Wolfgang, Dichgans Martin, Paquet Dominik

Abstract excerpt

The recent CRISPR revolution has provided researchers with powerful tools to perform genome editing in a variety of organisms. However, recent reports indicate widespread occurrence of unintended CRISPR-induced on-target effects (OnTEs) at the edited site in mice and human induced pluripotent stem cells (iPSCs) that escape standard quality controls. By altering gene expression of targeted or neighbouring genes,...

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PMID: 33597771
DOI: 10.1038/s41596-020-00481-2

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