Article

Optimization of gene knockout approaches and practical solutions to sgRNA selection challenges in hPSCs with inducible Cas9 system

2024-07-13

Abstract excerpt

<h4>Rationale</h4> CRISPR/Cas9 has been extensively used to knock out genes, allowing the study of genetic loss-of-function in human pluripotent stem cells (hPSCs). However, the current use of the Cas9-sgRNA plasmid or iCas9 system for gene editing in hPSCs has resulted in limited and inconsistent editing efficiency, as well as labor-intensive work. Additionally, identifying single-guide RNAs (sgRNAs) with high c...

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Literature Corpus work: d76a86ef-fbfb-5ed2-be5a-24c1fc0d9010
DOI: 10.1101/2024.07.09.602644

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