Minimal genome-wide human CRISPR-Cas9 library

Abstract excerpt

CRISPR guide-RNA libraries have been iteratively optimised to provide increasingly efficient reagents, although their large size is a barrier for many applications. We designed an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9), by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared to other libraries while preserving assay sen...

Topics

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• Base Sequence
• CAR-T cell therapy research
• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• Genetic Variation
• High-Throughput Nucleotide Sequencing
• RNA, Guide, CRISPR-Cas Systems

Identifiers and source

Literature Corpus work

9a83c26f-f324-5eb8-8048-d6577791acc3

DOI

10.1101/848895