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Article

Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening.

Nature protocols - 1 Apr 2017

Joung Julia, Konermann Silvana, Gootenberg Jonathan S, Abudayyeh Omar O, Platt Randall J, Brigham Mark D, Sanjana Neville E, Zhang Feng

Abstract excerpt

Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. Recently, the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeats) immune system has been adapted for genome-scale screening by combining Cas9 with pooled guide RNA libraries. Here...

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Identifiers

PMID: 28333914
DOI: 10.1038/nprot.2017.016
PMCID: PMC5526071

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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