Article

Massively Targeted Evaluation of Therapeutic CRISPR Off-Targets in Cells

2022-03-17

Abstract excerpt

<title>Abstract</title> <p>Sensitive and high-throughput evaluation of CRISPR RNA-guided nucleases (RGNs) off-targets (OTs) in cells are essential for safety assessment and advancing RGN-based gene therapies. Here we apply a modified library approach, SURRO-seq, for simultaneously evaluating thousands of off-target sites for therapeutic RGNs in cells. The SURRO-seq captures RGN-induced indels in barcoded surrogat...

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Literature Corpus work: bc5dcc9b-4541-5199-a8c4-0a6e4c84fb63
DOI: 10.21203/rs.3.rs-1427273/v1

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