Article

A xeno-free protocol for rapid differentiation of human iPSC-derived microglia from the KOLF2.1J reference line

2025-08-01

Abstract excerpt

We present a detailed, xeno-free protocol for the rapid differentiation of human induced pluripotent stem cells (hiPSCs) into microglia using the well-characterized KOLF2.1J reference line. This system employs doxycycline-inducible expression of six transcription factors (6-TF), stably integrated into the CLYBL safe harbor locus, to drive uniform microglial differentiation within two weeks. Adapted from Dräger et...

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Literature Corpus work: 05a635bb-c5c5-5a3a-a735-61b926af6de5
DOI: 10.1101/2025.07.28.667242

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