Article
Evolution in vitro of an RNA enzyme with altered metal dependence.
Nature - 14 Jan 1993
Lehman N, Joyce G F
Abstract excerpt
The Tetrahymena group I ribozyme catalyses a sequence-specific phosphodiester cleavage reaction on an external RNA oligonucleotide substrate in the presence of a divalent metal cation cofactor. This reaction proceeds readily with either Mg2+ or Mn2+, but no detectable reaction has been reported w...
Topics
- Animals
- Base Sequence
- Biological Evolution
- Calcium
- Kinetics
- Magnesium
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligoribonucleotides
- RNA, Catalytic
- Substrate Specificity
- Tetrahymena