Article
Comparison of the hammerhead cleavage reactions stimulated by monovalent and divalent cations.
RNA (New York, N.Y.) - 1 Apr 2001
O'Rear J L, Wang S, Feig A L, Beigelman L, Uhlenbeck O C, Herschlag D
Abstract excerpt
Although the hammerhead reaction proceeds most efficiently in divalent cations, cleavage in 4 M LiCl is only approximately 10-fold slower than under standard conditions of 10 mM MgCl2 (Murray et al., Chem Biol, 1998, 5:587-595; Curtis & Bartel, RNA, 2001, this issue, pp. 546-552). To determine if the catalytic mechanism with high concentrations of monovalent cations is similar to that with divalent cations, we...
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