Specialization of the DNA-cleaving activity of a group I ribozyme through in vitro evolution.

doi:10.1006/jmbi.1996.0496

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Article

Specialization of the DNA-cleaving activity of a group I ribozyme through in vitro evolution.

Journal of molecular biology - 13 Sept 1996

Tsang J, Joyce G F

Abstract excerpt

In an earlier study, an in vitro evolution procedure was applied to a large population of variants of the Tetrahymena group I ribozyme to obtain individuals with a 10(5)-fold improved ability to cleave a target single-stranded DNA substrate under simulated physiological conditions. The evolved ri...

Topics

Animals
Base Sequence
DNA, Single-Stranded
Evolution, Molecular
Genotype
Hydrolysis
Molecular Sequence Data
Mutagenesis, Site-Directed
Nucleic Acid Conformation
Phenotype
RNA, Catalytic
Substrate Specificity
Tetrahymena

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