Article
In vitro selection for altered divalent metal specificity in the RNase P RNA.
Proceedings of the National Academy of Sciences of the United States of America - 23 Dec 1997
Frank D N, Pace N R
Abstract excerpt
The ribozyme RNase P absolutely requires divalent metal ions for catalytic function. Multiple Mg2+ ions contribute to the optimal catalytic efficiency of RNase P, and it is likely that the tertiary structure of the ribozyme forms a specific metal-binding pocket for these ions within the active-site. To identify base moieties that contribute to catalytic metal-binding sites, we have used in vitro selection to...
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