Article
Direct measurement of oligonucleotide substrate binding to wild-type and mutant ribozymes from Tetrahymena.
Proceedings of the National Academy of Sciences of the United States of America - 1 Nov 1990
Pyle A M, McSwiggen J A, Cech T R
Abstract excerpt
Like protein enzymes, RNA enzymes (ribozymes) provide specific binding sites for their substrates. We now show that equilibrium dissociation constants for complexes between the Tetrahymena ribozyme and its RNA substrates and products can be directly measured by electrophoresis in polyacrylamide g...
Topics
- Animals
- Base Sequence
- Binding, Competitive
- Kinetics
- Magnesium
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- RNA, Catalytic
- Tetrahymena