Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA.

Abstract excerpt

The discovery of RNA enzymes has, for the first time, provided a single molecule that has both genetic and catalytic properties. We have devised techniques for the mutation, selection and amplification of catalytic RNA, all of which can be performed rapidly in vitro. Here we describe how these techniques can be integrated and performed repeatedly within a single reaction vessel. This allows evolution experiments...

Topics

• Animals
• Base Sequence
• Catalysis
• DNA, Single-Stranded
• Gene Amplification
• Introns
• Kinetics
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation
• RNA
• RNA Precursors