Article
Directed evolution of an RNA enzyme.
Science (New York, N.Y.) - 31 Jul 1992
Beaudry A A, Joyce G F
Abstract excerpt
An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic function. A population of 10(13) variants of the Tetrahymena ribozyme, a group I ribozyme that catalyzes sequence-specific cleavage of RNA via a phosphoester transfer mechanism, was generated. This enzyme h...
Topics
- Animals
- Base Composition
- Base Sequence
- Catalysis
- DNA, Single-Stranded
- Genotype
- Hot Temperature
- Magnesium Chloride
- Molecular Sequence Data
- Mutagenesis
- Mutagenesis, Site-Directed
- Phenotype
- Polymerase Chain Reaction
- RNA, Catalytic
- Substrate Specificity
- Tetrahymena thermophila