Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Directed evolution of an RNA enzyme.

Science (New York, N.Y.) - 31 Jul 1992

Beaudry A A, Joyce G F

Abstract excerpt

An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic function. A population of 10(13) variants of the Tetrahymena ribozyme, a group I ribozyme that catalyzes sequence-specific cleavage of RNA via a phosphoester transfer mechanism, was generated. This enzyme has a limited ability to cleave DNA under conditions of high temperature or high MgCl2 concentration, or both. A...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Composition
Share to Base Sequence
Share to Genotype

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Molecular Sequence Data

Share to Mutagenesis

Share to Mutagenesis, Site-Directed

Share to Phenotype

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 1496376
DOI: 10.1126/science.1496376

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Directed evolution of an RNA enzyme.PMID 1496376Read ↗

Specialization of the DNA-cleaving activity of a group I ribozyme through in vitro evolution.Semantic proximity Read ↗

Evolution in vitro: analysis of a lineage of ribozymes.Semantic proximity Read ↗

Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA.Semantic proximity Read ↗

Evolutionary optimization of the catalytic properties of a DNA-cleaving ribozyme.Semantic proximity Read ↗

Replicability and recurrence in the experimental evolution of a group I ribozyme.Semantic proximity Read ↗

In vitro evolution of a ribozyme that contains 5-bromouridine.Semantic proximity Read ↗

The genotypic landscape during in vitro evolution of a catalytic RNA: implications for phenotypic buffering.Semantic proximity Read ↗

Witnessing the structural evolution of an RNA enzyme.Semantic proximity Read ↗

Evolution in vitro of an RNA enzyme with altered metal dependence.Semantic proximity Read ↗

In vitro genetic analysis of the Tetrahymena self-splicing intron.Semantic proximity Read ↗

Forty years of in vitro evolution.Semantic proximity Read ↗

Isolation of new ribozymes from a large pool of random sequences [see comment].Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.