Article
Role of the CDR1 region of the TCR beta chain in the binding to purified MHC-peptide complex.
International immunology - 1 Oct 1994
Lone Y C, Bellio M, Prochnicka-Chalufour A, Ojcius D M, Boissel N, Ottenhoff T H, Klausner R D, Abastado J P, Kourilsky P
Abstract excerpt
Single alanine substitutions were introduced into the CDR1 region of the beta chain of a Kd-restricted TCR. Mutants and wild-type TCR were attached to the zeta chain of the CD3 complex and expressed at the surface of a rat basophil cell line. Transfectants were tested for the binding of purified soluble Kd-peptide complexes. With this experimental system, accessory molecules are unlikely to play a major role and...
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