Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system.

The Journal of reproduction and development - 13 Dec 2024

Lin Qingyi, Takebayashi Koki, Torigoe Nanaka, Liu Bin, Namula Zhao, Hirata Maki, Tanihara Fuminori, Nagahara Megumi, Otoi Takeshige

Abstract excerpt

CRISPR/Cas9-based multiplex genome editing via electroporation is relatively efficient; however, lipofection is versatile because of its ease of use and low cost. Here, we aimed to determine the efficiency of lipofection in CRISPR/Cas9-based multiplex genome editing using growth hormone receptor (GHR) and glycoprotein alpha-galactosyltransferase 1 (GGTA1)-targeting guide RNAs (gRNAs) in pig zygotes. Zona...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR-Cas Systems
Share to Fertilization in Vitro

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Galactosyltransferases

Share to Gene Editing

Share to Lipids

Share to Receptors, Somatotropin

Share to RNA, Guide, CRISPR-Cas Systems

Share to Zygote

Source

Open in PubMed Open full text

Identifiers

PMID: 39218670
DOI: 10.1262/jrd.2024-054
PMCID: PMC11658923

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system.PMID 39218670Read ↗

Evaluation of culture methods and chemical reagent combinations on CRISPR/Cas9 gene editing systems by lipofection in pig zygotes.Semantic proximity Read ↗

One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs.Semantic proximity Read ↗

Generation of mutant pigs by lipofection-mediated genome editing in embryos.Semantic proximity Read ↗

Effects of centrifugation treatment before electroporation on gene editing in pig embryos.Semantic proximity Read ↗

Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes.Semantic proximity Read ↗

Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.Semantic proximity Read ↗

Suppression of mosaic mutation by co-delivery of CRISPR associated protein 9 and three-prime repair exonuclease 2 into porcine zygotes via electroporation.Semantic proximity Read ↗

Efficient biallelic mutation in porcine parthenotes using a CRISPR-Cas9 system.Semantic proximity Read ↗

Efficient production of biallelic <i><scp>GGTA</scp>1</i> knockout pigs <i>by</i> cytoplasmic microinjection of <scp>CRISPR</scp>/Cas9 into zygotesSemantic proximity Read ↗

GEEP Method: An Optimized Electroporation-Mediated Gene Editing Approach for Establishment of Knockout Pig Lines.Semantic proximity Read ↗

Optimization Strategy for Generating Gene-edited Tibet Minipigs by Synchronized Oestrus and Cytoplasmic Microinjection.Semantic proximity Read ↗

Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.