Article
Suppression of mosaic mutation by co-delivery of CRISPR associated protein 9 and three-prime repair exonuclease 2 into porcine zygotes via electroporation.
The Journal of reproduction and development - 14 Feb 2020
Yamashita Shiro, Kogasaka Yuhei, Hiradate Yuuki, Tanemura Kentaro, Sendai Yutaka
Abstract excerpt
Gene-modified animals, including pigs, can be generated efficiently by introducing CRISPR associated protein 9 (CRISPR/Cas9) into zygotes. However, in many cases, these zygotes tend to become mosaic mutants with various different mutant cell types, making it difficult to analyze the phenotype of gene-modified founder animals. To reduce the mosaic mutations, we introduced three-prime repair exonuclease 2 (Trex2),...
Topics
- Animals
- Blastocyst
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Electroporation
- Embryonic Development
- Exodeoxyribonucleases
- Gene Editing