Article
GEEP Method: An Optimized Electroporation-Mediated Gene Editing Approach for Establishment of Knockout Pig Lines.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Tanihara Fuminori, Hirata Maki, Otoi Takeshige
Abstract excerpt
Pigs are excellent large animal models owing to their several physiological and anatomical similarities to humans. Somatic cell nuclear transfer using gene-modified cells is the mainstream approach for generating genetically modified pigs. Recent advances in improving gene editors such as the CRISPR/Cas9 system have enabled direct gene modification in zygotes/embryos. Here, we describe the gene editing by...
Topics
- Humans
- Animals
- Swine
- Gene Editing
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Mutation
- Zygote
- Electroporation