Article
Optimization Strategy for Generating Gene-edited Tibet Minipigs by Synchronized Oestrus and Cytoplasmic Microinjection.
International journal of biological sciences - 1 Jan 2019
Chen Bangzhu, Gu Peng, Jia Junshuang, Liu Wei, Liu Yumin, Liu Wen, Xu Tao, Lin Xiaolin, Lin Taoyan, Liu Yu, Chen Hengwei, Xu Mingchen, Yuan Jin, Zhang Jianing, Zhang Yinghui, Xiao Dong, Gu Weiwang
Abstract excerpt
The Tibet minipig is a rare highland pig breed worldwide and has many applications in biomedical and agricultural research. However, Tibet minipigs are not like domesticated pigs in that their ovulation number is low, which is unfavourable for the collection of zygotes. Partly for this reason, few studies have reported the successful generation of genetically modified Tibet minipigs by zygote injection. To...
Topics
- Animals
- CRISPR-Cas Systems
- Cytoplasm
- Estrus Synchronization
- Female
- Gene Editing
- Male
- Microinjections
- Mutation
- Superovulation