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Article

Effects of centrifugation treatment before electroporation on gene editing in pig embryos.

In vitro cellular & developmental biology. Animal - 1 Aug 2024

Liu Bin, Wittayarat Manita, Takebayashi Koki, Lin Qingyi, Torigoe Nanaka, Namula Zhao, Hirata Maki, Nagahara Megumi, Tanihara Fuminori, Otoi Takeshige

Abstract excerpt

Genetic mosaicism, characterized by multiple genotypes within an individual, is considered an obstacle to CRISPR/Cas9 genome editing in animal models. Despite the various strategies for minimizing mosaic mutations, no definitive methods exist to eliminate them. This study aimed to enhance gene editing efficiency in porcine zygotes using CRISPR/Cas9, which targets specific genes through centrifugation and zona...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 38833208
DOI: 10.1007/s11626-024-00926-y

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026.

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