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Article

Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes.

BMC biotechnology - 18 Aug 2020

Tanihara Fuminori, Hirata Maki, Nguyen Nhien Thi, Sawamoto Osamu, Kikuchi Takeshi, Doi Masako, Otoi Takeshige

Abstract excerpt

BACKGROUND: Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection. GGTA1-modified pigs, therefore, are promising donors for pig-to-human xenotransplantation. In this study, we developed a method for the...

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Identifiers

PMID: 32811500
DOI: 10.1186/s12896-020-00638-7
PMCID: PMC7436961

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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