Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection.

doi:10.1111/xen.12131

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Article

Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection.

Xenotransplantation - 1 Jan 2000

Li Ping, Estrada Jose L, Burlak Christopher, Montgomery Jessica, Butler James R, Santos Rafael M, Wang Zheng-Yu, Paris Leela L, Blankenship Ross L, Downey Susan M, Tector Matthew, Tector A Joseph

Abstract excerpt

BACKGROUND: Manipulating the pig genome to increase compatibility with human biology may facilitate the clinical application of xenotransplantation. Genetic modifications to pig cells have been made by sequential recombination in fetal fibroblasts and liver-derived cells followed by cross-breeding or somatic cell nuclear transfer. The generation of pigs for research or organ donation by these methods is slow,...

Topics

Animals
Antigens, Heterophile
Biotinylation
CRISPR-Cas Systems
Female
Galactosyltransferases
Gene Deletion
Gene Knockout Techniques
Genetic Vectors
Hepatocytes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Immunomagnetic Separation

Mixed Function Oxygenases

Nuclear Transfer Techniques

Phenotype

Plant Lectins

Pregnancy

RNA, Guide, CRISPR-Cas Systems

Streptavidin

Source

Open in PubMed

Identifiers

PMID: 25178170
DOI: 10.1111/xen.12131

Genes

GGTA1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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