Article
Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
International journal of molecular sciences - 4 Dec 2017
Sato Masahiro, Miyoshi Kazuchika, Nakamura Shingo, Ohtsuka Masato, Sakurai Takayuki, Watanabe Satoshi, Kawaguchi Hiroaki, Tanimoto Akihide
Abstract excerpt
The recent advancement in genome editing such a CRISPR/Cas9 system has enabled isolation of cells with knocked multiple alleles through a one-step transfection. Somatic cell nuclear transfer (SCNT) has been frequently employed as one of the efficient tools for the production of genetically modified (GM) animals. To use GM cells as SCNT donor, efficient isolation of transfectants with mutations at multiple target...
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