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Article

One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs.

In vitro cellular & developmental biology. Animal - 1 Sept 2020

Hirata Maki, Wittayarat Manita, Tanihara Fuminori, Sato Yoko, Namula Zhao, Le Quynh Anh, Lin Qingyi, Takebayashi Koki, Otoi Takeshige

Abstract excerpt

In the present study, we investigated whether electroporation could be used for one-step multiplex CRISPR/Cas9-based genome editing, targeting IL2RG and GHR in porcine embryos. First, we evaluated and selected guide RNAs (gRNAs) by analyzing blastocyst formation rates and genome editing efficiency. This was performed in embryos electroporated with one of three different gRNAs targeting IL2RG or one of two gRNAs...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 32978715
DOI: 10.1007/s11626-020-00507-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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