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Evaluation of culture methods and chemical reagent combinations on CRISPR/Cas9 gene editing systems by lipofection in pig zygotes.

In vitro cellular & developmental biology. Animal - 1 Aug 2024

Lin Qingyi, Takebayashi Koki, Torigoe Nanaka, Liu Bin, Namula Zhao, Hirata Maki, Nagahara Megumi, Tanihara Fuminori, Otoi Takeshige

Abstract excerpt

The delivery of CRISPR/Cas ribonucleoprotein (RNP) complexes is gaining attention owing to its high cleavage efficiency and reduced off-target effects. Although RNPs can be delivered into porcine zygotes via electroporation with relatively high efficiency, lipofection-mediated transfection appears to be versatile because of its ease of use, low cost, and adaptation to high-throughput systems. However, this system...

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PMID: 38664280
DOI: 10.1007/s11626-024-00908-0

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