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Article

Generation of mutant pigs by lipofection-mediated genome editing in embryos.

Scientific reports - 13 Dec 2021

Hirata Maki, Wittayarat Manita, Namula Zhao, Le Quynh Anh, Lin Qingyi, Takebayashi Koki, Thongkittidilok Chommanart, Mito Taro, Tomonari Sayuri, Tanihara Fuminori, Otoi Takeshige

Abstract excerpt

The specificity and efficiency of CRISPR/Cas9 gene-editing systems are determined by several factors, including the mode of delivery, when applied to mammalian embryos. Given the limited time window for delivery, faster and more reliable methods to introduce Cas9-gRNA ribonucleoprotein complexes (RNPs) into target embryos are needed. In pigs, somatic cell nuclear transfer using gene-modified somatic cells and the...

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Identifiers

PMID: 34903813
DOI: 10.1038/s41598-021-03325-5
PMCID: PMC8668999

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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