Article
Recurrent Genetic Abnormalities in Human Pluripotent Stem Cells: Definition and Routine Detection in Culture Supernatant by Targeted Droplet Digital PCR.
Stem cell reports - 14 Jan 2020
Assou Said, Girault Nicolas, Plinet Mathilde, Bouckenheimer Julien, Sansac Caroline, Combe Marion, Mianné Joffrey, Bourguignon Chloé, Fieldes Mathieu, Ahmed Engi, Commes Thérèse, Boureux Anthony, Lemaître Jean-Marc, De Vos John
Abstract excerpt
Genomic integrity of human pluripotent stem cells (hPSCs) is essential for research and clinical applications. However, genetic abnormalities can accumulate during hPSC generation and routine culture and following gene editing. Their occurrence should be regularly monitored, but the current assays to assess hPSC genomic integrity are not fully suitable for such regular screening. To address this issue, we first...
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