Article

Recurrent genetic abnormalities in human pluripotent stem cells: definition and routine detection in culture supernatant by targeted droplet digital PCR

2019-06-03

Abstract excerpt

<h4>Summary</h4> Genomic integrity of human pluripotent stem cells (hPSCs) is essential for research and clinical applications. However, genetic abnormalities can accumulate during hPSC generation and routine culture and following gene editing. Their occurrence should be routinely monitored, but the current assays to assess hPSC genomic integrity are not fully suitable for suchregular screening. To address this i...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c9ab7d9f-a4e0-5f6c-a5a6-af45496186eb
DOI: 10.1101/658484

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Recurrent genetic abnormalities in human pluripotent stem cells: definition and routine detection in culture supernatant by targeted droplet digital PCRDOI 10.1101/658484Read

Recurrent Genetic Abnormalities in Human Pluripotent Stem Cells: Definition and Routine Detection in Culture Supernatant by Targeted Droplet Digital PCR.Semantic proximity Read

Culture-acquired genetic variation in human pluripotent stem cells: Twenty years on.Semantic proximity Read

Comprehensive Assessment of the Genomic Stability of Human Induced Pluripotent Stem Cells for Clinical ApplicationsSemantic proximity Read

Comprehensive assessment of the genomic stability of human induced pluripotent stem cells for clinical applications.Semantic proximity Read

Detecting Genetic Mosaicism in Cultures of Human Pluripotent Stem Cells.Semantic proximity Read

Substantial somatic genomic variation and selection for <i>BCOR</i> mutations in human induced pluripotent stem cellsSemantic proximity Read

<i>StemCNV-check</i> : a pipeline for human pluripotent stem cell (hPSC) genomic integrity control using SNP array data and copy number variant scoringSemantic proximity Read

Insights into the mutational burden of human induced pluripotent stem cells using an integrative omics approachSemantic proximity Read

Genomic analysis of hESC pedigrees identifies de novo mutations and enables determination of the timing and origin of mutational events.Semantic proximity Read

Acquired Genetic and Epigenetic Variation in Human Pluripotent Stem Cells.Semantic proximity Read

Droplet digital PCR for large genomic rearrangements detection: A promising strategy in tissue BRCA1 testingSemantic proximity Read

<i>De novo</i> cancer mutations frequently associate with recurrent chromosomal abnormalities during long-term human pluripotent stem cell cultureSemantic proximity Read